Chemistry Journal of Moldova

LC-MS ANALYSIS AND ANTIOXIDANT ACTIVITY OF THE HYDRO-ALCOHOLIC EXTRACT OF MELISSA OFFICINALIS L. FROM ALGERIA

Author(s):

Brahim Ben Aicha, Rachid Rouabhi, Salim Gasmi, Chawki Bensouici, Hichem Mohammedi, Imad Mennai


Field: Natural product chemistry and synthesis
Type: Research paper
Issue: 2020 Volume 15, no.2
Pages: 78-87
Keywords:

hydro-methanolic extract, Melissa officinalis L., LC-MS analysis, antioxidant activity.


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DOI: http://dx.doi.org/10.19261/cjm.2020.778

Abstract (PDF)
Supplementary Material (PDF)
Graphical Abstract: The present work focuses on evaluation of the chemical composition and antioxidant activity of the hydro-methanolic extract of Melissa officinalis from Algeria. The liquid chromatography-mass spectrometry analysis allowed the identification of six compounds: caffeic acid, caftaric acid, hydroxyjasmonic acid glucoside, caftaric acid glucoside, rosmarinic acid and sagerinic acid. The in-vitro antioxidant activity of the hydro-methanolic extract was evaluated by using four different methods including: radical scavenging assay (DPPH), scavenging activity (ABTS), cupric reducing antioxidant capacity, and ferric reducing power assay.  The extract exhibited a relatively strong antioxidant activity compared to the synthetic antioxidants. The highest radical scavenging activity was registered using DPPH and ABTS methods,  IC50= 20.53±2.64 μg/mL and 22.50±0.67 μg/mL, respectively. These results suggest that Melissa officinalis L. could be considered a potential source of natural antioxidants with potential interest in the agrochemical and pharmaceutical industries.


 


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